Composite

Part:BBa_K1336007:Design

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-10)


LacI Expression Cassette with BsDyP gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1741
    Illegal BsaI site found at 1750
    Illegal BsaI site found at 2716
    Illegal SapI site found at 2146
    Illegal SapI site found at 2504
    Illegal SapI.rc site found at 2660


Design Notes

This part was designed by ligating our BBa_K314103 with our preexisting part BBa_K1336003

Source

From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University.

References